I have carried out a DIABLO analysis integrating three blocks of variables against my outcome (0 = healthy, 1 = diseased).
However, the CircosPlot shows incongruent results when comparing expression lines and correlations between some pairs of variables.
For example, based on the expression lines (and confirmed by a univariate comparison beween groups), variable X is higher in group 0 than in group 1, while variable Y is higher in group 1 than in group 0. However, the correlation between these variables depicted as positive. When I extract the correlation matrix from the CircosPlot, the correlation between the two variables is indeed positive. Can you please explain how this is possible?