RNA expression data derived from RT-PCR in diablo

Analysis
1

I am performing multi-omics integration with DIABLO. I have an RNA dataset derived from qPCR. What is the correct data type to use? I used -delta ct values. Is it OK? or I should use for example, 2^-dct. Please let me know.

2

hi @zia

We won’t be able to give you advice on this. All we ask is for the data to be processed and normalised with your method of choice. Try both and look at whether the results make more sense one way, or the other!
And do let us know what was your decision - it might be useful for other users.

Kim-Anh

3

Thank you for your reply. There was no huge difference in the results, but I decided to use the 2^-dct values of RNA expression data, since it is a better way to represent data from qPCR.

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