Integration two dataset microbiome - Metabarcode

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1

Hi everyone,

I have two sets of ITS data and 16S rRNA (Illumina) and I would like to integrate an analysis to check if there is an association between them. I would like to know if this package can be used for this analysis. If yes, what the input I should to use (table ASV or some distance for example “Bray-curtis”).

I’m sorry for the questions simple.
Thank you for help.

2

hi @aoliveira,
Definitely use the count matrices, not the the diversity metric. See this pipeline to process your count data a bit: http://mixomics.org/mixmc/mixmc-pre-processing/

Consider applying the following methods:

  • PCA on each data set first
  • rCCA or PLS or sPLS and inspect the graphical outputs.

Good luck!
Kim-Anh

3

@kimanh.lecao
Thank you for help.
I’ll go to try this way.
Thanks,

Amanda