I want to perform differential abundance on my CLR-transformed relative abundance table (metaphlan output from Shotgun data). At first I was just extracting the top taxa based on the PERMANOVA coefficient, but I was also considering doing DESeq, since this is what I usually use for differential abundance analysis. However, I was usually using it with untransformed, raw counts, but for this data I only have relative abundance information. I was wondering if it makes sense to offset the CLR-transformed counts by the minimum negative value to make the values all positive and feed them to DESeq to treat something like raw counts? I was doing this and it returned me more or less the same taxa I got by PERMANOVA, but I wanted to know if this is something you would advise.