Hello,

I have a question about your mixOmics R package. Do you have any recommendations for what the “minimum sample size” should be for using PLS to integrate 2 data sources, or DIABLO to integrate 3 data sources? Or are you aware of any publications that give sample size recommendations for PCA or PLS?

My dataset has 3 data sources (each with about 10-20 variables), and I have 3 groups of subjects, each with N=10 (total N=30). I plan to only use unsupervised PLS and DIABLO and not use the supervised versions in order to help prevent over-fitting due to my smaller sample size. I’m hoping N=10 per group is enough to use mixOmics but I am not sure if this is a good idea.

Thanks,

Jaron