Number of items to replace is not a multiple of replacement length error in perf() function

Dear mixOmics team,

I am really excited by the package you have created and am currently trying to integrate transcriptomic, metabolomic and microbiomic data. I already ran through your tutorials and everything worked fine, but now I am trying to perform sPLS pairwise integration on my data sets, which have been imputed beforehand, and it seems like something is not quite right.

# Read in imputed data
# Transcriptomics
tran_imp <- read_excel("~/Documents/AIT/Transcriptomics/tran_mad_imp.xlsx")
tran_imp <-
rownames(tran_imp) <- tran_imp[,1]
tran_imp <- tran_imp[,-1]
# Metabolomics
meta_imp <- read_excel("~/Documents/AIT/Metabolomics/t0_metabolites.xlsx",sheet = 3)
meta_imp <-
rownames(meta_imp) <- meta_imp[,1]
meta_imp <- meta_imp[,-1]
# Microbiomics
mibi_imp <- read_excel("~/Documents/AIT/Microbiomics/asv_imp.xlsx")
mibi_imp <-
rownames(mibi_imp) <- mibi_imp[,1]
mibi_imp <- mibi_imp[,-1]

# Storability Classes
storability <- read_excel("~/Documents/AIT/Beetroot Files/Metatable_Multiomics.xlsx", sheet = 1, col_names = TRUE)
storability <- as.factor(storability$Storability)

dim(tran_imp) #28 10000
dim(meta_imp) #28 25
dim(mibi_imp) #28 4398

# Transcriptomics & Metabolomics
X <- tran_imp
Y <- meta_imp
cbind(rownames(X), rownames(Y)) 
      [,1]      [,2]     
 [1,] "V1_1_t0" "V1_1_t0"
 [2,] "V1_2_t0" "V1_2_t0"
 [3,] "V1_3_t0" "V1_3_t0"
 [4,] "V1_4_t0" "V1_4_t0"
 [5,] "V2_1_t0" "V2_1_t0"
 [6,] "V2_2_t0" "V2_2_t0"
 [7,] "V2_3_t0" "V2_3_t0"
 [8,] "V2_4_t0" "V2_4_t0"
 [9,] "V3_1_t0" "V3_1_t0"
[10,] "V3_2_t0" "V3_2_t0"
[11,] "V3_3_t0" "V3_3_t0"
[12,] "V3_4_t0" "V3_4_t0"
[13,] "V4_1_t0" "V4_1_t0"
[14,] "V4_2_t0" "V4_2_t0"
[15,] "V4_3_t0" "V4_3_t0"
[16,] "V4_4_t0" "V4_4_t0"
[17,] "V5_1_t0" "V5_1_t0"
[18,] "V5_2_t0" "V5_2_t0"
[19,] "V5_3_t0" "V5_3_t0"
[20,] "V5_4_t0" "V5_4_t0"
[21,] "V6_1_t0" "V6_1_t0"
[22,] "V6_2_t0" "V6_2_t0"
[23,] "V6_3_t0" "V6_3_t0"
[24,] "V6_4_t0" "V6_4_t0"
[25,] "V7_1_t0" "V7_1_t0"
[26,] "V7_2_t0" "V7_2_t0"
[27,] "V7_3_t0" "V7_3_t0"
[28,] "V7_4_t0" "V7_4_t0"

tranmeta.spls <- spls(X, Y, ncomp = 3)
tune.spls <- perf(tranmeta.spls, validation = "Mfold", folds = 5, progressBar = TRUE, nrepeat = 100)

The spls function works, on all but the microbiomics data, where it says “In cor(A[[k]], variates.A[[k]]) : Standard devation is zero”. That might be because of the many zeros in my ASV data.

The main thing that bothers me is that , the perf() function always gives me the error " number of items to replace is not a multiple of replacement length".
I suspect it might have something to do with my data sets, but I have checked them
multiple times now I am still none the wiser.

Transcriptomics: 28 subjects, 3 classes, 10000 genes
Metabolomics: 28 subjects, 3 classes, 25 metabolites
Microbiomics: 28 subjects, 3 classes, 4398 ASVs (with a lot of zeros)
Since the datasets were imputed, there are no missing values in them.

Do you might know what could be causing the problem or do you have any suggestions on what to try to make it work?
Thank you so much for your assistance and thank you again for all the work that has been done!

Hi @lisatucek,

Thank you for reporting this issue. Can you please send us the data so we can fix this issue?

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Thank you so much, Al. I sent you an email on the listed address.
Kind regards,