I was trying to do Log-transformation followed the link http://mixomics.org/mixmc/mixmc-pre-processing/ for so to continue analysis the PCA.
But return the error :
Error in clr.transfo(X, offset = offset) : make sure you use pseudo counts before normalisation to avoid 0 values with log ratio transformation
I need to transform the log always? Or I can to do the others steps? The error can be the type of the transformation?
Until step previous for to check heterogenity library size it worked.
Thank you so much