Thanks for developing this great package!!
I have six omics data (transcriptome, proteome, cytokines, metabolome, gut16s, nares16s) to integrate using DIABLO, each one of them is normalized by appropriate method according to its platform.
My first question is: should I also transform the data after the normalization step e.g for RNA-seq median of ratios normalization + variance stabilizing transformation? I am asking about this because my log2 transformed and normalized proteome data showed a better separation than the normalized data in the plotIndiv, however, I am afraid that the data will then be scaled in mixomics and if all of this will affect the data true values.
Note: some data after the normalization don’t follow normal distribution.
My second question is: if I have unbalanced groups do you recommend to randomly select equal number of each group or just should depend on the BER as my BER is high (~0.35).
Many thanks in advance